Performance validation of the bd accuri c6 was then done using 6 and 8peak fluorescent bead mixtures spherotech according to instructions bd accuri c6 software user guide. Jul 22, 2016 accuri c6 consumables consumables fluids. Cited in over 9,000 publications, the bd accuri c6 plus enables you to go beyond your current research potential. It features the flexibility of automatic or manual counting mode, multi user capability and a multitasking operation. Many bd accuri c6 cytometer users can begin collecting and analyzing data with the help of a quick start guide. This portable and compact system holds the analytical power of a cytometer twice its size. Additional cflow softare information can be found in the cflow users guide located. Many bd accuri c6 customers can begin collecting and analyzing data with the help of a quick start guide. Bd accuri c6 flow cytometer instrument manual 7820018 rev2 3 refer to the bd accuri c6 safety and limitations manual pn 7820091 for complete safety information. Operate the instrument as described in this instruction manual. Mar 10, 2021 bd biosciences accuri c6 plus flow cytometer. Breakthrough with the bd accuri c6 plus cell analyzer.
Plus has been copied to the cflow computer from the installation cd or the. Using the bd accuri c6 cytometer for rapid and accurate. Bd accuri c6 instrument manual pdf download manualslib. Bd accuri c6 plus software has an intuitive user interface. Open the bead template in the bd accuri c6 software. The bd accuri c6 plus personal flow cytometer is the newest generation of the bd accuri platform. A wide dynamic range of over 7 decades ensures that all data is available all of the time. Accuri technical support for assistance with other operating systems. Follow the installation instructions in the wizard. User adjustable flow rate and core diameter for a variety of. Clickfree c6 user manual bd accuri c6 software user guide manual. In addition, c6 has a flow metering system for automatic calculation of absolute cell counts. Analysis can be performed on the bd accuri c6 plus flow cytometer or can be exported into thirdparty programs. Run the validation beads pn 653144 or 653145 see the bd accuri c6 software user guide for details.
Numerous processes are automated, such as daily qc, and the intuitive software interface guides the user through workflows. Digital compensation the accuri c6 plus software does not come with an automatic. Among cytoflex and bd accuri c6 plus which one is best. For information on how to use the bd pyxis medstation es user guide, which provides medication management capabilities to the imx105 bd pyxis es refrigerator, see the link provided. The tabbed interface provides quick access to the collection, analysis and statistics functions.
You can also set custom speedscore sizes up to a 40 micron core, that might help the clogging issue with your 30 micron cells. I had an question as a future user of flow cytometer. Bd csampler installation manual pdf download manualslib. Refer to the bd accuri c6 safety and limitations manual pn 7820091 for complete. Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient. Anyone have experience with the new bd accuri c6 plus. The unique fluidics system of the bd accuri c6 plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. Any settings will be remembered between samples until they are changed by the user. Frequency task supplies and parts after every experiment run filtered di water at the fast rate for 3 to 5 minutes to prevent clogging. Whether you are a new user to bd pyxis ciisafe, use v7.
Bd accuri c6 plus flow cytometer from bd biosciences. The default configuration of the bd accuri c6 is similar to the facscalibur2 3blue 1red with filters optimized for the detection of fluorochromes such as fitc, pe. Figure 6 shows how the fcs file data will look when initially opened in flowjo. Any one have good or bad experiences with the new bd. Mesf values determined using thermo scientific cytocal multifluor plus. The following directions illustrate how to zoom in on this area. Finally, the plant dna analysis template was opened. Bd accuri c6 with plate handler april 2016 this protocol contains instructions for setting up an accuri c6 with plate handler.
All users are responsible for obtaining the proper approval and training before the use of the bd accuri c6 flow cytometer. Samples and controls can be collected in any position on the plate in the software. Msds linked at the right cleaning solution, kr225 citric acid. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on youtube. Standard fluorochrome in use are identical to facs calibur. Bd bactec lytic10 anaerobicf culture vials soybeancasein digest broth in a plastic vial. Instrument manual bd accuri c6 instrument manual 31 pages. The bd totalys system offers a unique balance of automation and customization, streamlining processes and removing inefficiencies at every step of the cervical cancer screening process, from collection to result. Figure 6 shows how the fcs file data will look when initially opened in. When shutting down an instrument for long termstorage, perform standard cleaning or decontamination.
Bd csampler software user guide iv 782009901 rev2 table of figures figure 11. The system includes blue and red lasers, four color detectors, and both forward and side scatter detectors plus software that is so intuitive that you will typically be up and running within. The accuri csampler is an optional accessory for the c6 flow cytometer that. Rather than reading a good book with a cup of tea in the afternoon, instead they juggled with some harmful virus inside their computer. To fill the cleaning solution bottle green line, add 3 ml of 1030% citric acid and fill with di. From the instrument menu in bd accuri c6 software, activate the manual decontamination fluid cycle by selecting run decontamination fluid. The bd accuri c6 flow cytometer an affordable, fullfeatured, easytouse flow cytometer. Accuri c6 sample is not taken for analysis and overflow. The bd accuri c6 plus offers simplified workflows and readytouse templates. Bd bactec mycof lytic culture vials supplemented middlebrook 7h9 and brain heart infusion broth for use with bd bactec fluorescent series instruments.
Even new flow cytometry users find it so easy to learn and use that. Default cytometer configuration for the accuri c6 analyzer. Bd accuri c6 with single tube loader protocol this protocol contains instructions for setting up an accuri c6 with tube loader. The accuri cflow or cflow plus software cflow allows you to control the c6. See bd accuri c6 software user s guide, chapter 1, the basics for a complete description of how to run and analyze bead results. Di water daily if the cytometer is not shut down run the decontamination and cleaning cycles. If you are the first user of the day, you can run for 15 minutes. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers.
We have 1 bd accuri c6 manual available for free pdf download. The bd accuri c6 flow cytometer system includes bd accuri c6 software for controlling the instrument, generating statistics, and analyzing results. Bd accuri c6 software user guide manual vi 782009501 rev0 table b1. Bd accuri c6 cytometer manual college of medicine accuri c6 user guide, but end up in infectious downloads. Bd accuri c6 plus software files can be exported in fcs 3. Bd life sciences diagnostic systems package inserts bd.
The bd accuri c6 plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the singlecell level. Bd accuri c6 flow cytometer instruction manual, serial number 2400 and higher. Catalog number bd accuri c6 plus this product is no longer available on biocompare. Doubleclick the cflow or cflow plus icon on the desktop. Bd accuri c6 plus flow cytometer optical filter guide. Bd accuri c6 flow cytometers, bd cflow software and workstation pc supply all the capabilities of a fullfeatured flow cytometer at an affordable price. All users are responsible for the proper use, according to defined protocols, when using the bd accuri c6 flow cytometer. Take a leap in flow cytometry with the bd accuri c6 plus. The intuitive interface of the software guides the user through workflows. Bd accuri c6 plus software features an intuitive user interface. Refer to bd accuri c6 software user guide, chapter 1. Additional cflow softare information can be found in the cflow users guide l. Deionised water is recommended in the c6 manual try this and it may stop blockages. Allows the user to adjust the core stream diameter for a range of cell sizes.
Buy from accuri, or make your own citric acid solution. Additional laser configurations 2blue 2red, 4blue are available with the selectable lasers module cat. Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Accuri cytometers c6 flow cytometer community, manuals. Bd accuri c6, c6 plus, and bd facsvia cytometers perform these procedures when shutting down your instrument for long termstorage one week or more, unless otherwise specified in the instructions for use or user guide. Since yeasts are relatively large i use an accuri c6 to count bacteria so yeast are large to me i would go with the fasterlarger core size. The bd accuri c6 plus only weighs only 30 pounds and has a compact footprint of 11 x 14. Using the validation beads provided by accuri, run 50,000 events from each tube on the slow setting and save the data as todays date bead file. Even more colors to choose from with selectable lasers and filter replacement. A wide dynamic range of over 7 full decades ensures that all data is available all of the time.
Check calendar shut down instrument if youre the last user. S to help ensure that we can effectively serve our customers and impacted areas during this time of unprecedented demand, we are working to significantly increase our production capacity, and we have placed all bd alaris plus infusion pumps on manual inventory allocation until further notice for. Many bd accuri c6 plus cytometer users can begin collecting and analyzing data with the help of a quick start guide. It also includes common troubleshooting help and service information. The bd csampler is an optional accessory for the bd accuri c6 flow cytometer that allows for the collection of samples prepared in 48 and 96well plates and. Turn on the computer and log in with your ucf info. Direct counts on the bd accuri c6 plus correlate highly with counting beads and are more precise than manual counts. The bd accuri c6 is a digital basic flow cytometer, which is equipped with a blue 488nm and a red 640nm lasers, two light scatter detectors fsc and ssc, and four fluorescence detectors.
Accuri c6 software automatically displays the percentage of cells within the region. The software for accuri c6 cflow plus is very user friendly and efficient. Accuri c6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to facscalibur. Core research instrumentation school of pharmacy and. Filter maintenance and fluids were used as denoted in manuals. A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. By default, bd accuri c6 software displays the area parameter signified with a suffix of a, but parameters can also be displayed in height, width.
For detailed procedures, see the bd accuri c6 flow cytometer instrument manual. This manual provides information on cytometer requirements and specifications, setup, and maintenance. Install, update, and run antivirus protection software on a regular basis. Accuri cytometers c6 flow cytometer community, manuals and. Only operate the accuri c6 flow cytometer with an original ed version of software authorized by accuri.
Warning failure to keep the mat clear of plates, tubes or any other materials may result in damage to the bd csampler. Any one have good or bad experiences with the new bd accuri. Please contact us to schedule time on the instrument, set up a training appointment or to discuss a future project. Enhanced sensitivity, reliability, and capabilities bring flow. Accuri c6 is a digital pc based bench top analyser, 2 laser system. Bd pyxis ciisafe supply technologies clean and safety.
C6 flow cytometer instrument manual bd accuri c6 software user guide manual. By default, bd accuri c6 software displays the area parameter signified with a suffix of a, but parameters can also be displayed in height, width of primary threshold channel, only, or time. Hi as far as i know, there is nothing that bd can do to overcome this issue on accuri c6 itself by bd claims that they have used larger valves and thicker tubing system in accuri c6 plus and facsvia ivd version to cope with this problem. Bd pyxis es refrigerator imx105 operation user manual 360393 rev b this manual is for end users of the imx105 bd pyxis es refrigerator. Bd bd accuri c6 plus community, manuals and specifications.
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